C-26 Cell Culture and Inoculation -Quantified through cell counters -Regulated measurement growth at 37 degrees Celsius - Daily examination and measurement regarding tumor size,loss in body mass,tumor lesions,and body score conditions Resistance Training -Standard height cages (12.5 cm) for 8 weeks -Increased to 15.5 cm for a week -Final increase to 18 cm -Standard food and water throughout experiment Data Collection First 3 weeks-> daily measurement of food,body mass,and water Reduced to 3 days/week Body mass measurements weekly Post training-> returned to standard cage height Tissue Collection Final body mass collected post sedation Sedated using isoflurane Gastrocnemius extracted and weighed Frozen in isopentane Cooled in liquid nitrogen Stored at -80 degrees celsius Stained using Hematoxylin and Eosin Staining Procedure Tracing Used to measure individual muscle fibers Software-ImageJ Quantified-counted Adjusted-edited to make images lining clearer Measured to scale of 20 micrometers